Method and implantation coordinates into striatum.

<p>(<b>A</b>) Paxinos coordinates chosen for striatal infusion in mice aiming the centre of the left striatum at AP: +0.62 mm; ML: −1.75 mm to bregma a v d DV: −3.5 mm to the skull. The injection rate was 0.25 μl/hour for both experimental times. (<b>B</b>) Image of the implantation point in the mouse brain. (<b>C, D</b>) Methylene blue staining in the striatum, after 15 and 30 days of pump implantation. (<b>E</b>) HD mouse with pump installed subQ on the back and the brain infusion kit fixed with methacrylate to the skull. (<b>F</b>) High-affinity [³H]DA uptake in GCM “fresh” (immediately thawed), GCM incubated at 37° C, for 15 days compared with Defined medium (DM). The values are expressed as the mean ± SEM (n = 5). Statistical analysis was performed by one-way ANOVA, followed by Newman–Keuls multiple comparison test. ***p<0.001 GCM <i>vs</i> DM.