Alignment of the protein sequences of <i>S.</i><i>mansoni</i> sirtuins catalytic domains with orthologues from other species.

<p>Sequences used in the alignment of sirtuin class I (Figure A) in addition to SmSirt1 and SmSirt2 are <i>Clonorchis sinensis</i> Sirt1 (CsSirt1 Accession No: GAA56043.1) Sirt2 (CsSirt2 GAA29763.1), <i>Homo sapiens</i> Sirt1 (HsSirt1: AAH12499.1) Sirt2 (HsSirt2: NP_036369.2), <i>Drosophila melanogaster</i> Sirt1 (DmSirt1: NP_477351.1) Sirt2 (DmSirt2: NP_650880.2), <i>Caenorhabditis elegans</i> Sirt1 (CeSirt1: NP_001255484.1) Sirt2 (CeSirt2: NP_001024673.1). Sequences used in the alignment of sirtuin class III (Figure B) in addition to SmSirt5 are <i>C. sinensis</i> Sirt5 (CsSirt5: GAA47482.1), <i>H. sapiens</i> Sirt5 (HsSirt5: NP_036373.1), <i>Aplysia californica</i> Sirt5 (AcSirt5: NP_001191422.1), <i>Camponotus floridanus</i> Sirt5 (CfSirt5: EFN62888.1). Sequences used in the alignment of sirtuin class IV (Figure C) in addition to SmSirt6 and SmSirt7 are <i>C. sinensis</i> Sirt7 (CsSirt7: GAA293.65.2), <i>H. sapiens</i> Sirt6 (HsSirt6: CAG33481.1) Sirt7 (HsSirt7: NP_057622.1), <i>Xenopus laevis</i> Sirt6 (XlSirt6: NP_001085592.1), <i>C. floridanus</i> Sirt6 (CfSirt6: EFN65179.1) Sirt7 (CfSirt7: EFN70308.1), <i>D. melanogaster</i> Sirt6 (DmSirt6: NP_649990.2) Sirt7 (NP_651664.2). Solid lines above the sequence alignment indicate which regions of the proteins compose the Rossmann-fold domain (red), cofactor binding loop (black), small domain (blue) and loop regions (green). Highlighted residues in the sequence alignment indicate which amino acids are involved in NAD+ binding (green), zinc binding (blue) and acetyl-lysine peptide binding (red).