Increased gastritis and decreased <i>H. pylori</i> colonization in TLR2KO mice.

<p>C57BL/6 mice (n = 10 per group) were orally challenged with <i>H</i>. <i>pylori</i> SS1 (10⁹ CFU/mL) 3 times in 1 week starting on day 0. Mice were analyzed after 8 weeks. <b>a</b>, Schematic representation of the animal study. <b>b</b>, Micrographs of gastric histology (arrow pointing to area of inflammatory infiltrate). <b>c</b>, Gastric score was determined in a blinded fashion. <b>d</b> and <b>e</b>, Neutrophilic infiltration is increased in TLR2KO mice. Confocal immunofluorescent detection of MPO (green) and DAPI (blue) in the gastric fundus of <i>H</i>. <i>pylori</i>-infected WT versus TLR2KO mice. Morphometric analysis of MPO+ cells was measured and expressed as mean ± S.E.M. per 20x filed view. <b>f</b>, <i>H</i>. <i>pylori</i> colonization was determined by quantitative PCR of H. pylori 16S and <i>H</i>. <i>pylori</i>–Ura/<i>H</i>. <i>pylori</i> 16S mRNA. Results are showin as means ± S.E.M.. ^*<i>P</i> < 0.05 and ^**<i>P</i> < 0.01 compared with WT mice.