Intracellular cAMP levels in FSHRwt, FSHRmt, and FSHRwt/mt cells following treatment with FSH.

<p>Intracellular cAMP levels in 293T cells transfected with empty vector, FSHRwt, FSHRmt, or FSHRwt/mt followed by treatment without and with FSH at 10 mIU/mL (FSH 10) or 100 mIU/mL (FSH 100). Results were obtained with the cAMP-Glo Max assay kit and are shown as ∆relative light unit (∆RLU), the difference between treatment with and without FSH. Data expressed the mean ± SEM, obtained from three independent experiments. Single and double asterisks show the significant difference compared to MOCK cells treated with 10 mIU/mL and 100 mIU/mL of FSH, respectively (<i>P</i> <0.05). Two symbols, § and ¶, show the significant difference compared to FSHRmt cells treated with 10 mIU/mL and 100 mIU/mL of FSH, respectively (<i>P</i> <0.05).