The putative SRE motif located in the intragenic enhancer of <i>miR-1-1/133a-2</i> was verified by the chromatin immunoprecipitation (ChIP) assay with sequencing assessment <i>in vivo</i>.

<p>The cell chromatins were extracted from C2C12 cells transfected with either SRF expression vector or siRNA specific for SRF, and the SRF-SRE complexes were pulled down by the anti-SRF antibody. <b>A</b>. The oligonucleotides containing the SRF-binding site were detected (left panel). The PCR product was sequenced and the sequence matched with the enhancer (right panel). <b>B</b>. Quantitative PCR showed that SRF knockdown was achieved. <b>C</b>. Quantitative ChIP assays showed that loss of SRF resulted in decreases in the SRF-SRE complexes, and non-specific PCR primers failed to detect the SRE motif. SRE-P: specific primers for SRE; NS-P: non-specific primers upstream and downstream of the SRE site.