Antioxidant enzyme activities.

<p>This was observed in roots of wheat seedlings during 4°C stress at 0, 6, 12, 24, 48, 72 h and recovering 25°C for 24 h (total 96 h). <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0073380#s3" target="_blank">Results</a> were expressed as the mean of three replicates (n = 3, 0.1 g fresh roots from 5–10 seedlings in each replicate). Activities of <b>a</b>. superoxide dismutase (SOD), <b>b</b>. catalase (CAT), <b>c</b>. glutathione peroxidase (GSH-Px) and <b>d</b>. peroxidase (POD) in roots of wheat seedlings were examined using assay kits purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). CC+4°C, treated with 20 µg/mL CC under cold stress; CK+4°C, treated with 0.5% DMSO under cold stress; CK+25°C treated with 0.5% DMSO in growth chamber at 25°C.