XMRV reporter gene expression and virion production.

<p>A) Flow cytometry analysis of LNCaP cells transfected with <i>env</i> intact or <i>env</i> defect XMRV-GFP proviral DNA. B) GLUC activities in the supernatants of LNCaP cells transfected with <i>env</i> intact or <i>env</i> defect XMRV-GLUC constructs. C) Reverse transcriptase activities and D) Genomic RNA copy numbers (D) in supernatants of LNCaP cells transfected with XMRV wt, XMRV-GFP and XMRV-GLUC proviral DNA. E) qPCR and F) <i>Gaussia</i> luciferase activities of serial dilutions of an XMRV-GLUC virus stock. Values shown are average values derived from triplicate measurements ± standard deviation. Abbreviations and symbols: RLU/s (relative light units per second), n.s. (not significant), E (medium only), * (p<0.001), ** (p<0.00001).