Description of cucumber candidate reference genes based on the comparison with their Arabidopsis orthologs.

<p>Seven of the twelve candidate cucumber reference genes (<i>CACS</i>, <i>TIP41</i>, <i>PDF2</i>, <i>GW881873</i>, <i>YSL8</i>, <i>HEL</i>) have been recently retrieved from the whole cucumber genome sequence <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072887#pone.0072887-Migocka1" target="_blank">[36]</a> using the novel reference genes identified in <i>Arabidopsis</i> as the query sequences <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072887#pone.0072887-Czechowski1" target="_blank">[3]</a>. The commonly used remaining five genes (<i>ACT</i>, <i>TUA</i>, <i>UBI-1</i>, <i>EFα</i>, <i>CYP</i>) were previously available in the Genbank database as partial cDNAs. The full cDNAs and exon/intron organization of all 12 candidate genes were established using BlastN, and FGENESH or FGENESH+.