Uptake of propidium iodide (PI) by ACT-treated CHO and CR3⁺CHO-K1 cells.

<p>CHO cells were treated with 35-CR3 cells with 5 nM ACT for different incubation times (5, 10, 30, 60 and 120 min), then washed, trypsinized and incubated with propidium iodide for 4 min. PI uptake by the different cells was determined by flow cytometry as described in Materials and Methods section. Population of viable cells which show PI staining were gated in a FSH <i>vs</i> PI density plot, and propidium iodide positive cells were enumerated; data are displayed as fold activation over untreated controls with data-points representing mean values of at least three independent experiments ± SEM of at least three independent experiments, with **p<0.025, ***p<0.001.