Peptides R, I, and S inhibit the growth of primary human SN12C-pEGFP tumors.

<p>6 to 8-week-old female CD1 nude mice were injected subcutaneously with 2×10⁶ pEGFP-SN12C cells pre-treated with 10 µM AMD3100 or 10 µM of each peptide. Beginning the following day mice were further treated intraperitoneally for 10 days with 1.25 mg/kg AMD3100 or 2 mg/kg of peptides R, I or S. A week later mice were euthanized and tumor volume evaluated directly from the flank of mice using a Leica MacroFluo fluorescence stereomicroscope (Leica microsystem, Houston, TX) (A). The experiments were repeated three times. B. SN12C-PEGFP cells migration toward CXCL12 in the presence of AMD3100 or Peptide R, S and I.