Overview of high-throughput RNA-seq library preparation.

<p>mRNA is isolated from total RNA and fragmented. The mRNA is used to make first and second strands of cDNA and this double stranded cDNA molecules are subsequently synthesized, end-repaired and adenylated. Illumina adaptors are ligated to the processed double-stranded DNA and size selected. Finally, the ligated samples are then enriched by amplification using adapter specific primers and purified for sequencing.