<p>A heat activated (30 min, 100°C) spore was germinated at 65°C with 0.1 mM L-valine in 10 mM sodium phosphate buffer (pH 8.0), and the spore was monitored by Raman spectroscopy and DIC microscopy as described in Methods. Time-lapse Raman spectra of the trapped spores after the addition of L-valine were shown in (A). The indicated peaks at 661, 825, 1,017, 1,395 and 1,575 cm<sup>−1</sup> are the CaDPA bands. Normalized intensities of the CaDPA band at 1017<sup>−1</sup> (○) and DIC images (□) as the function of incubation time were shown in (B). The CaDPA band intensities and DIC image intensities were normalized to their initial values right after the addition of L-valine, and the DIC image intensity at 25 min was normalized to 0. The inte...
<p>Spores were activated by various methods, and germinated at 65°C with 1.0 mM L-valine and 10 mM s...
Dual-trap laser tweezers Raman spectroscopy (LTRS) and elastic light scattering (ELS) were used to i...
Spore-forming bacteria are a special problem for the food industry as some of them are able to survi...
Geobacillus stearothermophilus is a gram-positive, thermophilic bacterium, spores of which are very ...
Geobacillus stearothermophilus is a gram-positive, thermophilic bacterium, spores of which are very ...
Geobacillus stearothermophilus is a gram-positive, thermophilic bacterium, spores of which are very ...
The simultaneous nutrient germination of hundreds of individual wild-type spores of three Bacillus s...
Endospore-forming bacteria are associated with food spoilage, food poisoning, and infection in hospi...
Raman tweezers and quantitative differential interference contrast (DIC) microscopy are combined to ...
<p>Heat activated (30 min, 100°C (a,b), or unactivated spores (c,d) were germinated at 65°C with 1 m...
Raman scattering spectroscopy and elastic light scattering intensity (ESLI) were used to simultaneou...
<p>Heat activated spores (30 min, 100°C) were germinated at various temperatures with 1 mM L-valine ...
<p>Curve d in Fig. 1(C) is the Raman spectrum of single <i>B. cereus</i> spores that had lost their ...
Micro-Raman spectroscopy combined with optical tweezers is a powerful method to analyze how the bioc...
Bacterial spores are one of the most resilient life forms on Earth, and they can remain viable for a...
<p>Spores were activated by various methods, and germinated at 65°C with 1.0 mM L-valine and 10 mM s...
Dual-trap laser tweezers Raman spectroscopy (LTRS) and elastic light scattering (ELS) were used to i...
Spore-forming bacteria are a special problem for the food industry as some of them are able to survi...
Geobacillus stearothermophilus is a gram-positive, thermophilic bacterium, spores of which are very ...
Geobacillus stearothermophilus is a gram-positive, thermophilic bacterium, spores of which are very ...
Geobacillus stearothermophilus is a gram-positive, thermophilic bacterium, spores of which are very ...
The simultaneous nutrient germination of hundreds of individual wild-type spores of three Bacillus s...
Endospore-forming bacteria are associated with food spoilage, food poisoning, and infection in hospi...
Raman tweezers and quantitative differential interference contrast (DIC) microscopy are combined to ...
<p>Heat activated (30 min, 100°C (a,b), or unactivated spores (c,d) were germinated at 65°C with 1 m...
Raman scattering spectroscopy and elastic light scattering intensity (ESLI) were used to simultaneou...
<p>Heat activated spores (30 min, 100°C) were germinated at various temperatures with 1 mM L-valine ...
<p>Curve d in Fig. 1(C) is the Raman spectrum of single <i>B. cereus</i> spores that had lost their ...
Micro-Raman spectroscopy combined with optical tweezers is a powerful method to analyze how the bioc...
Bacterial spores are one of the most resilient life forms on Earth, and they can remain viable for a...
<p>Spores were activated by various methods, and germinated at 65°C with 1.0 mM L-valine and 10 mM s...
Dual-trap laser tweezers Raman spectroscopy (LTRS) and elastic light scattering (ELS) were used to i...
Spore-forming bacteria are a special problem for the food industry as some of them are able to survi...