Westernblot analysis of p40 phox after leptin stimulation.

<p>Neutrophils were stimulated with different concentrations of leptin for four(IEF strip pH 4–7 linear 13 cm) and 2D gels were blotted. (A) Representative example of a 2D westernblot stained for p40 phox and β-tubulin. (B–D) Quantification of spot volume corrected for β-tubulin and normalized against the 0 ng/ml leptin control, for p40 phox spot 1 (B), spot 2 (C) and spot 3 (D). Data are expressed as mean ± SEM, n = 3–8. Statistics done using one-way ANOVA with Bonferroni posttest comparing leptin stimulated to control (0 ng/ml leptin). (E) Representative example of a 2D westernblot stained for phosphorylated p40-phox (F,G) Intracellular luminol luminescence was measured in the context of different concentration of leptin. Neutrophils were not stimulated (E) or stimulated with STZ (F). data are expressed as mean ± SEM, n = 4–5. Statistics done using one-way ANOVA with Bonferroni posttest comparing leptin stimulated to control.