TonEBP is not involved in salt-dependent chemotaxis of macrophages.

<p>Western blot analysis of TonEBP protein expression with or without excess 40(wt) and TonEBP overexpressing (TonEBP overexp) RAW264.7 cells, respectively (A). Kinetics of cell migration of TonEBP overexpressing and wildtype RAW264.7 macrophages toward 40 mM excess NaCl over 20 hours using transwell migration assays (B). Western blot analysis of TonEBP expression in RAW264.7 cells following RNAi of TonEBP using lipofection for 72 hours; mock, lipofection without siRNA; ns siRNA, nonspecific control siRNA (C). Transwell migration assay of RAW264.7 cells following RNAi of TonEBP toward 40 mM excess NaCl (D). For all western blot analysis (A, C), β-actin protein expression was used as a loading control. The migration index (B, D) was determined by the number of transmigrated cells in relation to CXCL12-stimulated cells. “+”indicates that the stimulus was added to the lower well of the transwell (D). All cell migration data shown represent mean ± SD from 3 experiments performed in duplicate. ***p<0.001 as compared to cells without stimulus. n.s. not significant. n = 3.