RT-PCR analysis of <i>CfRXR</i>s expression.

<p>Transcripts of the four <i>CfRXR</i> isoforms were detected at the differentiating stage of both ovaries and testes (A), and in mantle, gill, digestive gland, and adductor muscle in sexually mature male and female scallops (B). For each of the developing stage of gonad and other tissues, RT-PCR was performed in three individuals. On the gel showing RT-PCR products of <i>CfRXR</i>s, from top to bottom, the four bands in a single lane correspond to <i>CfRXRd</i>, <i>CfRXRc</i>, <i>CfRXRb</i>, and <i>CfRXRa</i>, respectively. The <i>β-actin</i> cDNA fragments (<i>Cfβ-actin</i>) were amplified as internal control.