Copy number amplifications in chromosome 5 are specific for malignant adrenocortical tumors.

<div><p>A) Unsupervised euclidean distance genomic clustering (complete linkage) in 46 adrenocortical tumor samples (defined as sample ID). Best separation between adenoma (ACA, n=24, white) and carcinoma (ACC, n=22, black) obtained analyzing only the chromosome 5.</p> <p>B) Validation with FISH analysis of the copy number alterations detected with SNP array in chromosome 5. Two representative adrenocortical carcinomas (ACC, samples ID 456C and 1023C) with gains/amplifications at SNP array analysis validated by FISH (probe FISH_5q/5p-Probe: CSF1R/D5S23, D5S721). Gain of the red signal (specific probe) with respect to the green signal (centromer-specific reference probe) in more than 50% of cells. In black the ACC patients affected by ACC and in white the patients affected by ACA.</p>