11a induces minimal cell apoptosis in ovarian cancer cell lines and a breast cancer cell line.

<p>SKOV3, A2780 and OVCAR3 ovarian cancer cells (A) and MCF7 breast cancer cells (C) were treated with the indicated doses of 11a or doxorubicin for 24 hours. Total cell lysates were probed for PARP cleavage using anti-PARP antibody in western blots. β-Actin was used as a loading control. The black arrows indicate positions of non-cleaved and cleaved PARP proteins. (B) and (D) After 24 hours treatment with 2 µM 11a, cells were collected and stained with Annexin V/PI and subjected to flow cytometry. 50 µM etoposide (B) or 1 µM staurosporine (STS) (D) served as positive controls for apoptosis. The statistical significance were shown as **p<0.01, ***p<0.001 compared with DMSO control.