Intergenic sRNA igMAV_2868–2869 and its predicted homologs in <i>M. ulcerans</i> and <i>M. tuberculosis</i>.

<p>(A) Alignment of the intergenic region, MAV_2868–MAV_2869, coding for sRNA with its homologs in <i>M. ulcerans</i> (MUL_3032–3033) and <i>M. tuberculosis</i> (rv1846c–1847). Only regions coding for sRNA and their promoters are shown. Alignment of DNA sequences was performed with UGENE software <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0074209#pone.0074209-Okonechnikov1" target="_blank">[52]</a>. The promoter regions containing –35 and –10 sequences and the coding regions are conserved among the 3 species. Alignment of sRNAs and their consensus secondary structures was performed with the WAR webserver <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0074209#pone.0074209-Fortin1" target="_blank">[45]</a> and is shown below the alignment. Nucleotides that are conserved among the 3 species and form duplex structures are highlighted in green. (B) Predicted consensus secondary structure of igMAV_2868–2869. Extensive complementary regions form the secondary structure with very low free energy.