Epigenetic regulation of EGR-1 gene expression by APP.

<p><b>A</b>. Fragmented chromatin obtain from APP+/+ and APP-/- neurons DIV5 (n=3) was immunoprecipitated with antibody recognizing normal mouse IgG as negative control (IgG), anti-acetylated histone H3 (H3Ac) or anti-acetylated histone H4 (H4Ac). Quantification was realized by real-time qPCR performed on immunoprecipitated DNA with primers designed on EGR-1 promoter, and normalized to the input. n.s. non-significant. ***p<0.001. <b>B</b>. EGR-1 mRNA levels were quantified in APP+/+ and APP-/- neurons DIV5 treated (TSA) or not (NT) for 24 h with 25ng/ml TSA (n=6). Values were normalized to the GAPDH mRNA, and expressed as percentage of APP+/+. Two way Anova. **p<0.005, ***p<0.001. Relative induction of Egr-1 after exposure to TSA after normalization. Student t test. *p<0.05.