Sinomenine down-regulated RANKL-induced

<div><p>NF-κB <b>activation</b>. </p> <p>RAW264.7 cells, stably or transiently expressing NF-κB-luc reporter gene construct, were stimulated with RANKL for 8 h to study the effects of SIN on (A) stable or (B) transient NF-κB transcription. In another experiment, RAW264.7 cells were pretreated with SIN for 30 min and stimulated with 100 ng/ml RANKL for 30 min to (C) perform Western blot analysis of nuclear p65 and IκBα or (D) to observe p65 translocation to nuclear by fluorescence image. (C) The translocation of p65 by Western blot was expressed by dividing the nuclear p65 density with cytoplamic p65 density, the fold change of IκBα was normalized to β-actin. Densitometric quantification and statistical analysis include the results from 3 independent experiments. *P<0.05; **P<0.01 (compared with RANKL group); # P<0.05, # #P<0.01 (compared with control group).</p>