Perfusion Computed Tomography (CTP) analysis method.

<p>Cerebral blood volume (CBV) maps (coronal plane) from two rats taken immediately after intraluminal thread occlusion of the middle cerebral artery (MCAo). The animal in column A is seen to have MCAo with preserved AChA blood volume whereas the animal shown in column B has both MCAo and anterior choroidal artery occlusion (AChAo). For each rat, 2 bregma levels are shown, with the top 2 images showing the more caudal sections. At each level the colour coded CBV maps are shown on top, and underneath a CBV threshold of 10 ml/100 g was applied to all CBV maps to remove large blood vessels, and ROIs encompassing the left (contralateral, yellow outline) and right (ipsilateral, blue outline) hemispheres were generated. Voxels below the mean contralateral CBV were thresholded out to leave the voxels with higher CBV (white). Green arrows indicate the location of the AChA territory. Lack of CBV voxels in the ipsilateral AChA territory (column B, row 2) indicates occlusion of the AChA. For illustrative purposes, a CBV threshold of 2 ml/100 g <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0075779#pone.0075779-Wintermark1" target="_blank">[19]</a> has been applied to the CBV maps to indicate the areas predicted to infarct within the MCA territory (red).