Effect of verapamil on the accumulation and retention of Rhodamine 123 in <i>L. donovani</i> isolates.

<p>A. Accumulation under normal conditions after 1h loading (hatched bar), accumulation in presence of verapamil (black bar). B. Retention of Rho 123 after 1 h of efflux (hatched bar), retention in presence of verapamil at the time of accumulation (white dotted bar), retention in presence of verapamil at the time of efflux (black bar). Results are mean of three independent experiments performed from three different promastigote cultures. * P ≤ 0.05, ** P ≤ 0.005, *** P ≤ 0.0005 indicate statistical significance with respect to reference sensitive isolate S1 for resistant isolates (R1- R5) and strain Dd8 for lab resistant mutant Mt, ns indicates no statistically significant difference.