Methylation analysis of normal gastric tissues, primary gastric cancer tissues and peripheral blood from gastric carcinoma patients and healthy controls.

<p>(A) Online bioinformatics software was used to analyze the promoter region and the methylation status of the VEZT gene. (B) Methylation status of 34 CpG sites of the VEZT promoter from 30 normal gastric tissues and 30 primary gastric cancer tissues. Primary tumor tissues showed higher methylation levels in the VEZT promoter region when compared with normal gastric tissues. (C) Methylation status of 34 CpG sites of the VEZT promoter from peripheral blood plasma DNA of 30 gastric carcinoma patients and 30 healthy controls. Peripheral blood from gastric carcinoma patients showed higher methylation levels in VEZT promoter region when compared with healthy controls. Each row of circles represents an integrated methylation ratio from three clones, and each circle represents a single CpG site. Open circle represents unmethylated cytosine, whereas filled circles or partially filled circles represent the methylated ratio of CpG sites.