Immunoblotting of ABCB1/P-gp (A) and MRP7 (B) in HEK293 and HEK293/MRP7 cells and Immunofluorescence detection of MRP7 in HEK293/MRP7 cells (C).

<p>Cell lysates were prepared from HEK293/pcDNA3.1, HEK293/ABCB1 and HEK293/MRP7 cells (A), HEK293/MRP7 cells incubated with 5 µM BBA for different time periods (0, 24, 48 and 72 h) (B). Equal amounts (40 µg of protein) of total cell lysates were used for each sample. The localization of MRP7 by immunofluorescence was done on paraformaldehyde fixed cells using polyclonal antibody D19 against MRP7 and Alexa Flour^® 488 donkey anti-goat IgG(C). DAPI was used for nuclear counterstaining. Results from a representative experiment are shown. Similar results were obtained in two other trials.