IL-17A and a combination of IL-21/IL-23/IL-33 potentiate the ability of venom to induce the differentiation of IgG producing-ASC.

<p>Representation of the B cell differentiation in an <i>in </i><i>vitro</i> model. Purified CD19-positive Bmem (1.5 x 10⁵ cell/mL) obtained 48 d after venom immunization were cultured in a three-step in <i>vitro</i> model under basic conditions or in medium supplemented with V<i>Tn</i>, CpG or cytokines alone or in combination with venom for 9 d (<i>A</i>). Evaluation of intracellular content of IgG in CD138-positive ASC was determined by flow cytometry (<i>B</i>). The percentage of double-positive cells was analyzed in peritoneal (<i>C</i>), splenic (<i>D</i>) or medullar cells (<i>E</i>). The dashed line represents the percentage of IgG^pos CD138^pos ASC differentiated from CD19-positive B cells from control group of mice cultured in medium under basic conditions. #<i>p</i> < 0.05 compared to CD19-positive B cells from V<i>Tn</i>-immunized mice in medium under basic conditions; and ξ<i>p</i> < 0.05 compared to CD19-positive B cells from V<i>Tn</i>-immunized mice in medium supplemented with V<i>Tn</i>. Data are mean ± SEM values from three independent experiments. Dot plots are representative of 3 experiments.