COX-2-PGE₂ loop mediated the accumulation of CD11b+Gr1+ cells.

<p>BALB/c mice were subjected to 5-days restraint stress or left untreated. In some experiments, mice were i.p injected with SC-236 or same volume of solvent DMSO one hour before each restraint session. (A) Peripheral blood was sampled and serum PGE₂ and VEGF levels were analyzed by ELISA. Results are expressed as mean ± SD, n=5. (B) Bone marrow Gr1+ cells were purified and subjected to immunoblotting, n=3. One representative of two independent experiments with similar results is shown. Densitometric readings are shown for COX-2 and normalized with β-actin (set the lowest ratio as 1.0). (C) Bone marrow cells were subjected to flow cytometry for CD11b and Gr1 staining. Percentages of CD11b+Gr1+ cells were shown. Results are expressed as mean ± SD, n=5.