Effects on the macrophage phenotype by secreted factors from CRC cells.

<p>(A) Morphological evaluation of macrophages stimulated with conditioned media (Cm) from RKO, SW480 or Caco2 cell lines. (B) Expression of extracellular markers was evaluated by immunostaining and flow cytometry in macrophages of M1 and M2 subtype or in macrophages stimulated with conditioned media (Cm) from CRC cell lines. Relative mean flourescense intensity (MFI) ± SD of three or more independent experiments is shown, where each sample was normalized against its respective isotype control and with the M-CSF stimulated control sample set as 1.