Sweat gland LRCs express Keratin 15 and contribute long term to the acinar SG structure.

<p>(A) K15 staining of sweat gland LRCs indicate positive K15 expression. (B) Fluorescent photo of K15CrePR/R26eYFP^RU palm containing YFP positive sweat glands after long term YFP activation. (C) Section of K15CrePR/R26tdTom^RU crossed onto K5TetOff/TreH2BGFP sweat glands after 4 weeks of chase with doxycycline followed by 2 days of RU treatment. (D) K14 basal layer staining co-localizes with GFP expression in K15-GFP transgenic sweat glands. (E) K18 lumenal marker staining co-localizes with K15-GFP expression in sweat glands. (F) FACS analysis of K15-GFP sweat glands demonstrates that approximately half of the K15-GFP positive cells are localized to the basal layer expressing α6 integrin. (G) Histology of X-Gal-treated K15CrePR/R26LacZ transgenic mice, blue stain indicates transgene expression for more than 6 months after RU activation in sweat glands. (H) K15 expression co-localizes with LRCs in the acinar sweat gland region.