NEDD9 regulates Vimentin and E-cadherin expression.

<p>Expression of EMT markers, Vimentin and E-cadherin, was examined by Western blotting. (A) Vimentin was down-regulated and E-cadherin was upregulated while NEDD9 was silenced in SiHa and HeLa cells. (B) Vimentin was upregulated and E-cadherin was down-regulated while NEDD9 was overexpressed in HaCaT cells. Results of Transwell assay showed that decreased cell invasion (C) and migration (D) by shRNA of NEDD9 were increased by siRNA against E-cadherin in SiHa and HeLa cells. Scale bar, 100 μm. (E) E-cadherin was upregulated and Vimentin was down-regulated by virtue of FAK inhibitor PF-228 or SRC inhibitor PP2 in HaCaT cells with exogenous NEDD9. (F) Results of immunoprecipitation showed that tyrosine-phosphorylated NEDD9 and total NEDD9 were not significantly regulated while E-cadherin was suppressed by siRNA in HaCaT cells.