Regulation of expressions of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) on pleural mesothelial cells (PMCs) <i>in vitro</i>.

<p>A non-malignant transformed mesothelial cell line Met5A cell (PMC line) or PMCs derived from tuberculous pleural effusion (TPE) were cultured in medium alone, designated cytokines, tuberculosis-specific antigens (ESAT-6/CFP-10), or 50% volume TPE in the absence or presence of anti‒IFN-γ or ‒IL-4 mAbs. After 48 h, PMCs were stained and detected by flow cytometry. (A) Representative cytometric histograms show ICAM-1 expression on PMC line and PMCs from TPE cultured in medium alone or stimulated by IFN-γ. White histograms indicate isotype controls. (B) Comparisons of ICAM-1 MFI ratios of PMCs are shown. ICAM-1 fluorescence intensity (MFI) ratios were calculated by dividing MFI of each group by MFI of control group, respectively. (C) Representative cytometric histograms show VCAM-1 expression on PMCs from TPE cultured in medium alone, stimulated by IL-4 or TGF-β. White histograms indicate isotype controls. (D) Comparisons of VCAM-1 MFI ratios of PMCs are shown. In panels B and D, the results are reported as mean ± SEM from 5 independent experiments. The comparisons were determined by Kruskal-Wallis one-way analysis of variance on ranks. *p < 0.05 compared with the corresponding controls.