NKT cells regulate ILC2 derived IL-5 production independently of alveolar macrophages.

<p>(A) Alveolar macrophages (AM) and NKT were FACS sorted from the lungs at the indicated days and analyzed for IL-33 mRNA via RT-PCR. Cells were pooled from >5 mice for each group at each day. (B) AM were analyzed for intracellular IL-33 protein at 12 d.p.i. in indicated mouse strains. (C) Representative FACS plots of intracellular IL-5 in c-kit⁺ ILC2 at 12 d.p.i. after 24 hour <i>ex vivo</i> culture with or without indicated cell depletion. (D) Frequency of IL-5+ c-kit⁺ ILC2 at 12 d.p.i. following <i>ex vivo</i> cell depletion after 24 hour culture. Data is representative of 3 experiments with 4–10 mice each. Bars = +/− SEM. **p<.01, ***p<.001.