Determination of % anti-FL Abs in serum of a dengue patient by VLP-capture ELISA.

<p>(A) Serial dilutions of the serum were subjected to a capture ELISA using DENV1 WT and mutant VLPs containing mutations in the FL epitope (W101A+F108A). The bar graph displaying results from an anti-E ELISA shows that comparable amounts of WT and mutant VLPs were added based on recognition of E by pooled human dengue-immune sera. % anti-FL Abs = [1 – endpoint titer to mutant VLPs/endpoint titer to WT VLPs]×100%. (B) The same serum was subjected to a capture ELISA using DENV2 WT and mutant VLPs (W101A+F108A). Data are presented as in (A). Data are means with standard deviation of duplicates from one representative experiment of two. For endpoint titers, only means are shown.