Transglycosylation activity of Gt-Mamy.

<p>(a) Transglycosylation of hydrolytic products of G4, G5 and acarbose onto G1/G2 (Lanes 1,2,3,4,5,6,7,8,9,10,11 indicate standards, Ac, G1+G5+E, G1+G4+E, G1+Ac+E, G2+G5+E, G2+G4+E, G2+Ac+E, G1+G4+EΔ, G1+G5+EΔ, G1+Ac+EΔ, respectively); (b) Transglycosylation of hydrolytic products acarbose onto methyl-α-D-glucopyranoside (MG), mannitol (ML)and xylitol (XL) [Lanes 1,2,3,4,5,6,7,8,9,10 indicate MG, ML, XL, Ac, Ac+MG+E, Ac+ML+E, Ac+XL+E, Ac+MG+EΔ, Ac+ML+EΔ, Ac+XL+EΔ, respectively; (c) Transglycosylation of hydrolytic products acarbose onto xylose (X), rhamnose (X) and sucrose (S) [Lanes 1,2,3,4,5,6,7,8,9,10 indicate X, R, S, Ac+X+E, Ac+R+E, Ac+S+E, Ac+X+EΔ, Ac+R+EΔ, Ac+S+EΔ] (Abbreviations: G1, G2, G3, G4, G5, Ac and PTS stands for glucose, maltose, maltotriose, maltotetraose, maltopentaose, acarbose and pseudotrisaccharide, respectively; arrows indicate transglycosylated products).