Immunodepletion of the Prp19 complex from <i>Xenopus</i> egg extracts does not compromise mitotic microtubule assembly in general.

<p><i>Xenopus</i> egg extracts arrested in metaphase were depleted of the Prp19 complex using a specific antibody (dePrp19); an unspecific IgG served as a control. (A) Spindle assembly around sperm nuclei was monitored in <i>Xenopus</i> egg extracts after a complete cell cycle. (B): Spindle assembly around chromatin beads was monitored in <i>Xenopus</i> egg extracts after a complete cell cycle. (A) -(B): Microtubules are shown in red, chromatin (DAPI) in blue. (C): Microtubule aster assembly upon Ran GTP addition was monitored in cycled egg extracts. Scale bars: 20 µm.