Primer extension analysis on unadducted and BPDE-adducted primers with alternate DNA polymerases.

<p> Polymerase extensions of 27t/11p^* or 11p were performed as described in MATERIALS and METHODS. In all cases, 10 fmol of enzyme was used. The primer to polymerase ratio was 5. U, S, and R represent unadducted, (+) and (-)<i>-anti-trans</i>-BPDE-adducted primers, respectively. The unadducted and adducted primers have different electrophoretic mobilities that result in products of different mobilities. Panels represent primer extension assays in the presence of the respective polymerase: HIV-1 RT(RT), DNA polymerase β (pol β), Sequenase (Seq), KF exo^- (KF exo^-). The template sequence on the right coincides with full-length products that can be obtained by utilizing an 11-mer primer. The formation of truncated products is unique only to the <i>R</i>-adducted primer extended by HIV-1 RT. In some instances, non-templated blunt-end additions are observed (e.g., HIV-1 RT).