Fos expression in the PVH and DMH/DA areas.

<p>Schematics from the Paxinos and Watson <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0075733#pone.0075733-Paxinos1" target="_blank">[36]</a> atlas and representative photomicrographs of coronal sections from the PVH [the anterior-posterior (AP) coordinate of −1.80 mm from Bregma] and DMH/DA area (AP of −3.12 mm) are shown. The treatment [intravenous LPS (0.5 mg/kg) or saline] and T_a (30 or 24°C) are indicated. Photomicrographs of brain sections from rats treated at 30°C are framed in red; sections from rats treated at 24°C are framed in blue. Fos-immunoreactive nuclei can be seen as black dots. For the PVH, the following substructures are marked in the schematic: the dorsal cap (PaDC), the lateral magnocellular and medial parvocellular subnuclei (PaLM and PaMP, respectively), and the ventral aspect (PaV); the third ventricle (3V) and the posterior aspect of the anterior hypothalamic area (AHP) are marked for orientation. For the DMH, the dorsal, compact, and ventral aspects (DMD, DMC, and DMV, respectively) are marked; the DA is also shown. The mammilothalamic tract (mt), fornix (f), and 3V are shown as “landmarks.” The section marked “24°C, LPS” includes only a narrow ventral portion of the mt.