The effect of EGF treatment on the subcellular distribution of claudins 1 and 3.

<p>Cell monolayers were grown on glass coverslips and processed for immunofluorescence analysis of claudin-1 and claudin-3 distribution after EGF treatment (48 h). The stained cells were viewed with confocal <b><i>FV10i-O or</i></b> Axio Observe.Z1 microscopes. (<b>A</b>) Caco-2 cells<b><i>; bar: 5 </i></b>µm. (<b>B</b>) HT-29 cells; bar: 10 µm. <i>Arrowheads</i>; punctual accumulation of claudins. <i>Ctr</i>; control.