<p>(A) Treg cells, sorted from HBZ-Tg or non-Tg mice, were cultured in the presence of IL-2 for 3 or 7 days. The expression of Foxp3 was analyzed by flow cytometry. (B) Sequential changes of the Foxp3<sup>−</sup> population are shown. (C) IFN-γ production of <i>ex vivo</i> cultured Foxp3<sup>+</sup> cells was evaluated by intracellular staining. Sorted Treg cells were cultured for 7 days, and then stimulated for 4 h with PMA/ionomycin and protein transport inhibitor. (D) Foxp3 expression of sorted CD4<sup>+</sup>CD25<sup>+</sup>GITR<sup>high</sup>thymocytes from HBZ-Tg mice.</p
<p>(A) Foxp3 protein and YFP expression in Treg cells among gated CD4<sup>+</sup>T cells from the sp...
<p>Spleens from mice of the two groups were collected 14 days post immunization, pooled, and their c...
<p>CD4<sup>+</sup>CD25<sup>+</sup> T cells from 12–15 week-old Sick NOD mice were cultured overnight...
<p>(<b>A</b>) Fresh isolated CD4<sup>+</sup>CD25<sup>+</sup> Tregs were stained with CD4 and Foxp3 a...
<div><p>(A) CD25<sup>−</sup>CD4<sup>+</sup> T cells were cultured for 6 d in the presence of TGF-β a...
<p>CD25<sup>+</sup>CD4<sup>+</sup> Tregs were isolated ex vivo from pooled spleen and LN single-cell...
<p>The percentage of Foxp3<sup>+</sup> Treg cells was significantly increased upon FTY720 induction ...
<p>(A) CD4<sup>+</sup>FoxP3.gfp<i><sup>−</sup></i> T cells from naïve OT-II.FoxP3.gfp mice were cult...
<p>CD4<sup>+</sup>CD25<sup>+</sup> T cells from 12–15 week-old B6 mice were cultured overnight in mu...
<p>(A) Foxp3 expression in freshly isolated CD4<sup>+</sup> T cells from the spleen, mesenteric lymp...
<p>A&B). Groups (n = 6) of B6 and TCR-δ<sup>-/-</sup> mice were immunized with IRBP<sub>1-20</sub>/C...
<p>CD4<sup>+</sup>CD25<sup>+</sup> T cells from 12–15 week-old B6 or Sick NOD mice were cultured ove...
<p>Splenocytes from 5c.c7 Rag<sup>–/—</sup>TCR transgenic mice (<b>a</b>) or WT C57BL/6 mice (<b>b-e...
<p>(A) Dot plots display eGFP and Foxp3 expression among the live CD4<sup>+</sup> gated DEREG cells....
<p>(<b>A</b>) 1×10<sup>6</sup> Thy1.1+ CD4+CD25− T cells were co-transferred with 1×10<sup>6</sup> T...
<p>(A) Foxp3 protein and YFP expression in Treg cells among gated CD4<sup>+</sup>T cells from the sp...
<p>Spleens from mice of the two groups were collected 14 days post immunization, pooled, and their c...
<p>CD4<sup>+</sup>CD25<sup>+</sup> T cells from 12–15 week-old Sick NOD mice were cultured overnight...
<p>(<b>A</b>) Fresh isolated CD4<sup>+</sup>CD25<sup>+</sup> Tregs were stained with CD4 and Foxp3 a...
<div><p>(A) CD25<sup>−</sup>CD4<sup>+</sup> T cells were cultured for 6 d in the presence of TGF-β a...
<p>CD25<sup>+</sup>CD4<sup>+</sup> Tregs were isolated ex vivo from pooled spleen and LN single-cell...
<p>The percentage of Foxp3<sup>+</sup> Treg cells was significantly increased upon FTY720 induction ...
<p>(A) CD4<sup>+</sup>FoxP3.gfp<i><sup>−</sup></i> T cells from naïve OT-II.FoxP3.gfp mice were cult...
<p>CD4<sup>+</sup>CD25<sup>+</sup> T cells from 12–15 week-old B6 mice were cultured overnight in mu...
<p>(A) Foxp3 expression in freshly isolated CD4<sup>+</sup> T cells from the spleen, mesenteric lymp...
<p>A&B). Groups (n = 6) of B6 and TCR-δ<sup>-/-</sup> mice were immunized with IRBP<sub>1-20</sub>/C...
<p>CD4<sup>+</sup>CD25<sup>+</sup> T cells from 12–15 week-old B6 or Sick NOD mice were cultured ove...
<p>Splenocytes from 5c.c7 Rag<sup>–/—</sup>TCR transgenic mice (<b>a</b>) or WT C57BL/6 mice (<b>b-e...
<p>(A) Dot plots display eGFP and Foxp3 expression among the live CD4<sup>+</sup> gated DEREG cells....
<p>(<b>A</b>) 1×10<sup>6</sup> Thy1.1+ CD4+CD25− T cells were co-transferred with 1×10<sup>6</sup> T...
<p>(A) Foxp3 protein and YFP expression in Treg cells among gated CD4<sup>+</sup>T cells from the sp...
<p>Spleens from mice of the two groups were collected 14 days post immunization, pooled, and their c...
<p>CD4<sup>+</sup>CD25<sup>+</sup> T cells from 12–15 week-old Sick NOD mice were cultured overnight...