Lack of Myosin-IIA impairs T cell motility on endothelial monolayers.

<p>Fluorescently-labeled control (green) and MyoIIA KO (red) T cells were perfused onto endothelial monolayers under flow and imaged by time-lapse fluorescence microscopy. A) Representative image of fluorescence overlay (green and red) on phase contrast image (grey) from the analysis of a 15 min time-lapse imaging of T cell crawling and TEM. The color-coded tracks show the migration paths of the T cells during the time-lapse. Scale bar = 40μm. B) Speed of control and MyoIIA KO T cells crawling on endothelial cell monolayers. Data are the average (±SEM) of the mean of 3 independent experiments. C) Displacement (shown as distance²/time) of control and MyoIIA KO T cells crawling on endothelial cell monolayers. Data are the average (±SEM) of the mean of 3 independent experiments.