IL-17 promotes NK cell recruitment and activation in the liver.

<p>(<b>A</b>) Serum ALT levels in NK cell depleted mice at indicated times post PolyI:C stimulation. IL-17 was neutralized or not at the time of PolyI:C injection. (<b>B,C</b>) Absolute number of NK cells (B) and CD69 positive NK cells (C) at indicated time points in the liver after PolyI:C treatment, neutralized of IL-17 or not. (<b>D</b>) Flow cytometry staining of killing associated molecules (NKG2D, IFN-γ, Gramzyme B, perforin) expressed in NK cells 8 hours post PolyI:C treatment. Plots were gated on NKp1.1+CD3- cells. (<b>E</b>) Percentage of NK cell cytotoxity isolated from mice liver 8 hours after PolyI:C stimulation against YAC-1 cells. IL-17 was neutralized with monoclonal antibodies or not during the hepatitis. Data shown are Means ± SD (A-C,E) or representative (D) from 3 independent experiments. *P<0.05 **P<0.01.