Box plots of ELISA data using sera from non-infected control patients, <i>F. hepatica</i>-infected patients, and patients with various parasitic diseases.

<p>A: ELISA using anti-total IgG as secondary antibody and B: ELISA using anti-IgG4 as secondary antibody. The dashed line represents the cut-off for negative samples. <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002414#s3" target="_blank">Results</a> are obtained from three independent experiments conducted in duplicate.