Analysis of ELISA data using FhCL1 antigen and secondary antibodies specific for IgG1, IgG2 and IgG4+IgG1 isotypes.

Differential immunolocalization of ES antigens in Fasciola gigantica as revealed by confocal microscopy, using anti-Fg ES hyperimmune sera and the FITC conjugated secondary antibody.

M. A. Hannan Khan (3713455)
Rizwan Ullah (770801)
Abdur Rehman (11154)
Lubna Rehman (630836)
Ahammed Shareef P. A. (4488175)
S. M. A. Abidi (3713452)

October 2017

Differential immunolocalization of ES antigens in Fasciola gigantica as revealed by confocal micr...

Comparative assessment of ELISAs using recombinant saposin-like protein 2 and recombinant cathepsin L-1 from Fasciola hepatica for the serodiagnosis of human Fasciolosis.

Gottstein, Bruno
Schneeberger, Marianne
Boubaker, Ghalia
Merkle, Bernadette
Huber, Cristina
Spiliotis, Markus
Müller, Norbert
Garate, Teresa
Doherr, Marcus

June 2014

Two recombinant Fasciola hepatica antigens, saposin-like protein-2 (recSAP2) and cathepsin L-1 (recC...

The Diagnosis of Human Fascioliasis by Enzyme-Linked Immunosorbent Assay (ELISA) Using Recombinant

Cathepsin L Protease
Bibiana Gonzales Santana
John P. Dalton
Fabio Vasquez Camargo
Michael Parkinson

August 2016

Background: Fascioliasis is a worldwide parasitic disease of domestic animals caused by helminths of...

Analysis of ELISA results using FhCL1 antigen and anti-total-IgG as secondary antibody.

Bibiana Gonzales Santana (460972)
John P. Dalton (182938)
Fabio Vasquez Camargo (460973)
Michael Parkinson (460974)
Momar Ndao (187600)

September 2013

A) Histogram of control and Fasciola-positive serum samples. B) Histogram of normalized to...

Scattergraphs for ELISA data using various secondary antibodies.

Bibiana Gonzales Santana (460972)
John P. Dalton (182938)
Fabio Vasquez Camargo (460973)
Michael Parkinson (460974)
Momar Ndao (187600)

September 2013

Data obtained using secondary antibodies specific for anti-IgG4 (A), anti-IgG2 (B) and anti-IgG1 ...

Sensitivity and specificity analysis for ELISA using anti-IgG4 as secondary antibody.

Bibiana Gonzales Santana (460972)
John P. Dalton (182938)
Fabio Vasquez Camargo (460973)
Michael Parkinson (460974)
Momar Ndao (187600)

September 2013

A) Histogram showing negative sample where two fall out of the cut-off limit. B) Histogram showin...

Indirect ELISA for Detection of Fascioliasis IgG Antibodies in Human Sera

Ramos Maldonado, Ernesto R.
Espino, PhD, Ana M.

August 2016

Currently, coprological examination based on egg detection in stool samples is used as the most idea...

Comparison of ELISA data obtained using secondary antibodies specific for various isotypes.

Bibiana Gonzales Santana (460972)
John P. Dalton (182938)
Fabio Vasquez Camargo (460973)
Michael Parkinson (460974)
Momar Ndao (187600)

September 2013

Absorbance between positive infected sera and negative control sera using anti-total IgG and the ...

Western blot analyses of recSAP2-antigen.

Bruno Gottstein (102082)
Marianne Schneeberger (577388)
Ghalia Boubaker (101987)
Bernadette Merkle (577389)
Cristina Huber (577390)
Markus Spiliotis (102093)
Norbert Müller (244550)
Teresa Garate (2889512)
Marcus G. Doherr (577391)

June 2014

Arrow indicates the 15 Mr size of the revealed recombinant and affinity-purified protein. Western...

Evaluation of the Stability of Coated Plates with Antigen at Different Temperatures and Times by ELISA Test to Diagnose Fasciolosis

MT Rahimi
S Koosha
M Aryaeipour
MB Rokni

February 2010

"nBackground: Considering that ELISA method presently is the test of choice for diagnosis of fasciol...

Western blot analysis of refolded proteins.

Abolfazl Mirzadeh (5814686)
Asiyeh Yoosefy (5814689)
Elham Kazemirad (5814692)
Zahra Barati (5814695)
Majid Golkar (5814698)
Jalal Babaie (5814701)
Farid Jafarihaghighi (5814704)
Zarrintaj Valadkhani (5814707)

October 2018

Reactivity of rtFhSAP-2 (1), rFhLAP (2) and rtFhFtn-1(3) proteins against serum samples of infected ...

Analysis of the expression of the chimeric recombinant protein rUS9-FhLAP in E. coli and optimization of its use in ELISA.

Ricardo A. Orbegozo-Medina (2818390)
Victoria Martínez-Sernández (340051)
María J. Perteguer (4608040)
Ana Hernández-González (317115)
Mercedes Mezo (340057)
Marta González-Warleta (340058)
Fernanda Romarís (340060)
Esperanza Paniagua (2818393)
Teresa Gárate (340055)
Florencio M. Ubeira (340062)

February 2019

(A) SDS-PAGE and WB analysis of rUS9-FhLAP expression. Lane 1: MW markers. Lane 2: Coomasie blue sta...

A reliable monoclonal antibody (MoAb)-based sandwich enzyme-linked immunosorbent assay (sandwich ELISA) is developed for diagnosis of fasciolosis by F. gigantica.

Panat Anuracpreeda (843520)
Runglawan Chawengkirttikul (843521)
Prasert Sobhon (177629)

January 2016

(A and B) The lowest concentrations of rCatL1, CatL1 in CE of variuos stages of F. giga...

Comparative assessment of ELISAs using recombinant saposin-like protein 2 and recombinant cathepsin L-1 from Fasciola hepatica for the serodiagnosis of human Fasciolosis

Gottstein, Bruno
Schneeberger, Marianne
Boubaker, Ghalia
Merkle, Bernadette
Huber, Cristina
Spiliotis, Markus
Müller, Norbert
Garate, Teresa
Doherr, Marcus G

June 2014

Two recombinant Fasciola hepatica antigens, saposin-like protein-2 (recSAP2) and cathepsin L-1 (recC...

Immunophenotypic profile of BM-MSCs (A) and CESCs (B) analyzed by FACS.

Lan-Tao Liu (5652757)
Bo Huang (30906)
Chang-Qing Li (5652760)
Ying Zhuang (341742)
Jian Wang (5901)
Yue Zhou (71752)

February 2013

The green lines represent the fluorescence intensity of cells stained with the indicated antibodi...

Differential immunolocalization of ES antigens in Fasciola gigantica as revealed by confocal microscopy, using anti-Fg ES hyperimmune sera and the FITC conjugated secondary antibody.

M. A. Hannan Khan (3713455)
Rizwan Ullah (770801)
Abdur Rehman (11154)
Lubna Rehman (630836)
Ahammed Shareef P. A. (4488175)
S. M. A. Abidi (3713452)

October 2017

Differential immunolocalization of ES antigens in Fasciola gigantica as revealed by confocal micr...

Comparative assessment of ELISAs using recombinant saposin-like protein 2 and recombinant cathepsin L-1 from Fasciola hepatica for the serodiagnosis of human Fasciolosis.

Gottstein, Bruno
Schneeberger, Marianne
Boubaker, Ghalia
Merkle, Bernadette
Huber, Cristina
Spiliotis, Markus
Müller, Norbert
Garate, Teresa
Doherr, Marcus

June 2014

Two recombinant Fasciola hepatica antigens, saposin-like protein-2 (recSAP2) and cathepsin L-1 (recC...

The Diagnosis of Human Fascioliasis by Enzyme-Linked Immunosorbent Assay (ELISA) Using Recombinant

Cathepsin L Protease
Bibiana Gonzales Santana
John P. Dalton
Fabio Vasquez Camargo
Michael Parkinson

August 2016

Background: Fascioliasis is a worldwide parasitic disease of domestic animals caused by helminths of...

Analysis of ELISA results using FhCL1 antigen and anti-total-IgG as secondary antibody.

Bibiana Gonzales Santana (460972)
John P. Dalton (182938)
Fabio Vasquez Camargo (460973)
Michael Parkinson (460974)
Momar Ndao (187600)

September 2013

A) Histogram of control and Fasciola-positive serum samples. B) Histogram of normalized to...

Scattergraphs for ELISA data using various secondary antibodies.

Bibiana Gonzales Santana (460972)
John P. Dalton (182938)
Fabio Vasquez Camargo (460973)
Michael Parkinson (460974)
Momar Ndao (187600)

September 2013

Data obtained using secondary antibodies specific for anti-IgG4 (A), anti-IgG2 (B) and anti-IgG1 ...

Sensitivity and specificity analysis for ELISA using anti-IgG4 as secondary antibody.

Bibiana Gonzales Santana (460972)
John P. Dalton (182938)
Fabio Vasquez Camargo (460973)
Michael Parkinson (460974)
Momar Ndao (187600)

September 2013

A) Histogram showing negative sample where two fall out of the cut-off limit. B) Histogram showin...

Indirect ELISA for Detection of Fascioliasis IgG Antibodies in Human Sera

Ramos Maldonado, Ernesto R.
Espino, PhD, Ana M.

August 2016

Currently, coprological examination based on egg detection in stool samples is used as the most idea...

Comparison of ELISA data obtained using secondary antibodies specific for various isotypes.

Bibiana Gonzales Santana (460972)
John P. Dalton (182938)
Fabio Vasquez Camargo (460973)
Michael Parkinson (460974)
Momar Ndao (187600)

September 2013

Absorbance between positive infected sera and negative control sera using anti-total IgG and the ...

Western blot analyses of recSAP2-antigen.

Bruno Gottstein (102082)
Marianne Schneeberger (577388)
Ghalia Boubaker (101987)
Bernadette Merkle (577389)
Cristina Huber (577390)
Markus Spiliotis (102093)
Norbert Müller (244550)
Teresa Garate (2889512)
Marcus G. Doherr (577391)

June 2014

Arrow indicates the 15 Mr size of the revealed recombinant and affinity-purified protein. Western...

Evaluation of the Stability of Coated Plates with Antigen at Different Temperatures and Times by ELISA Test to Diagnose Fasciolosis

MT Rahimi
S Koosha
M Aryaeipour
MB Rokni

February 2010

"nBackground: Considering that ELISA method presently is the test of choice for diagnosis of fasciol...

Western blot analysis of refolded proteins.

Abolfazl Mirzadeh (5814686)
Asiyeh Yoosefy (5814689)
Elham Kazemirad (5814692)
Zahra Barati (5814695)
Majid Golkar (5814698)
Jalal Babaie (5814701)
Farid Jafarihaghighi (5814704)
Zarrintaj Valadkhani (5814707)

October 2018

Reactivity of rtFhSAP-2 (1), rFhLAP (2) and rtFhFtn-1(3) proteins against serum samples of infected ...

Analysis of the expression of the chimeric recombinant protein rUS9-FhLAP in E. coli and optimization of its use in ELISA.

Ricardo A. Orbegozo-Medina (2818390)
Victoria Martínez-Sernández (340051)
María J. Perteguer (4608040)
Ana Hernández-González (317115)
Mercedes Mezo (340057)
Marta González-Warleta (340058)
Fernanda Romarís (340060)
Esperanza Paniagua (2818393)
Teresa Gárate (340055)
Florencio M. Ubeira (340062)

February 2019

(A) SDS-PAGE and WB analysis of rUS9-FhLAP expression. Lane 1: MW markers. Lane 2: Coomasie blue sta...

A reliable monoclonal antibody (MoAb)-based sandwich enzyme-linked immunosorbent assay (sandwich ELISA) is developed for diagnosis of fasciolosis by F. gigantica.

Panat Anuracpreeda (843520)
Runglawan Chawengkirttikul (843521)
Prasert Sobhon (177629)

January 2016

(A and B) The lowest concentrations of rCatL1, CatL1 in CE of variuos stages of F. giga...

Comparative assessment of ELISAs using recombinant saposin-like protein 2 and recombinant cathepsin L-1 from Fasciola hepatica for the serodiagnosis of human Fasciolosis

Gottstein, Bruno
Schneeberger, Marianne
Boubaker, Ghalia
Merkle, Bernadette
Huber, Cristina
Spiliotis, Markus
Müller, Norbert
Garate, Teresa
Doherr, Marcus G

June 2014

Two recombinant Fasciola hepatica antigens, saposin-like protein-2 (recSAP2) and cathepsin L-1 (recC...

Immunophenotypic profile of BM-MSCs (A) and CESCs (B) analyzed by FACS.

Lan-Tao Liu (5652757)
Bo Huang (30906)
Chang-Qing Li (5652760)
Ying Zhuang (341742)
Jian Wang (5901)
Yue Zhou (71752)

February 2013

The green lines represent the fluorescence intensity of cells stained with the indicated antibodi...

Differential immunolocalization of ES antigens in Fasciola gigantica as revealed by confocal microscopy, using anti-Fg ES hyperimmune sera and the FITC conjugated secondary antibody.

M. A. Hannan Khan (3713455)
Rizwan Ullah (770801)
Abdur Rehman (11154)
Lubna Rehman (630836)
Ahammed Shareef P. A. (4488175)
S. M. A. Abidi (3713452)

October 2017

Differential immunolocalization of ES antigens in Fasciola gigantica as revealed by confocal micr...

Comparative assessment of ELISAs using recombinant saposin-like protein 2 and recombinant cathepsin L-1 from Fasciola hepatica for the serodiagnosis of human Fasciolosis.

Gottstein, Bruno
Schneeberger, Marianne
Boubaker, Ghalia
Merkle, Bernadette
Huber, Cristina
Spiliotis, Markus
Müller, Norbert
Garate, Teresa
Doherr, Marcus

June 2014

Two recombinant Fasciola hepatica antigens, saposin-like protein-2 (recSAP2) and cathepsin L-1 (recC...

The Diagnosis of Human Fascioliasis by Enzyme-Linked Immunosorbent Assay (ELISA) Using Recombinant

Cathepsin L Protease
Bibiana Gonzales Santana
John P. Dalton
Fabio Vasquez Camargo
Michael Parkinson

August 2016

Background: Fascioliasis is a worldwide parasitic disease of domestic animals caused by helminths of...

Analysis of ELISA data using FhCL1 antigen and secondary antibodies specific for IgG1, IgG2 and IgG4+IgG1 isotypes.

Abstract

Extracted data

Analysis of ELISA data using FhCL1 antigen and secondary antibodies specific for IgG1, IgG2 and IgG4+IgG1 isotypes.

Abstract

Extracted data

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