Transactivation activity assay.

<p>Co-transfection of empty (Mock), wild-type (WT), or mutant (MT) Sf1 expression vectors and a <i>Amh</i> (A), <i>Inhibin-a</i> (B), <i>Cyp11a1</i> (C) or <i>Cyp19a1</i> (D) promoter reporter were performed in HEK293T cells. Results are expressed as a percentage of WT activity (RLU WT%). The potential dominant negative effect of the p.Y5D mutant was assessed by co-transfecting WT expression vector with empty or MT vector (1∶1) (A, B, C, D) and increasing MT (0, 10, 20, 30, 40 ng) with 10 ng empty (−) or 10 ng WT (+) vector (Amh only) (E) in HEK293T cells. ** P<0.01, *** P<0.001. RLU, relative light units.