Cytoskeletal disruption leads to a delay in the VEGFR2 response.

<p>(A) Endothelial cells treated with cytoskeletal inhibitors Nocodazole or CCD show a loss of immediate ERK1/2 phosphorylation (pERK1/2) after 5 minutes of VEGF stimulation compared to controls while total ERK1/2 levels remain constant. (B) Endothelial cells treated with cytoskeletal inhibitors, similar to controls, undergo a decrease in the total VEGFR2 levels after 60 minutes of VEGF stimulation. Panels (C) and (D) are data derived from 2 independent experiments and show the mean densitometry of these bands normalized to untreated control levels. Significant differences (denoted by asterisks) determined using paired student’s T-Test. Threshold of significance (alpha) was set to p<0.01. *p= 0.00014, **p= 0.00049.