Effect of leptin on HCT116 cells mitochondria.

<p>HCT116 cells were treated with DMEM (control) vs. leptin (100 ng/ml), for 24 hours. (<i>A</i>, <i>B</i>) Gene expression levels were detected using quantitative real-time PCR (<i>n</i>=4). *, <i>P</i>< 0.05, **, <i>P</i>< 0.01 vs. respective control of each gene (Student’s <i>t</i>-test). (<i>C</i>) Cell lysates were analyzed for cytochrome C (CytC, top panel) and β-actin (bottom panel) by Western blot, and densitometric analysis of the data was made. * <i>P</i>> 0.01, vs. control (Student’s <i>t</i>-test).