Reduction of proliferative neural progenitor cells in the nCT brain.

<p>A1-B2: BrdU pulse-labeled cells in the germinal zone were decreased in the LGE of E11.5 nCT brain (A2) compared to that in the nC brain (A1). Similar results were found in the MGE (B1, B2). C1–C2: Reduction of BrdU pulse-labeled cells in the germinal zone were also found in the LGE of E12.5 nCT brain (C2). The white double-headed arrows indicate the width of BrdU-positive germinal zone. D: Quantitative analysis of BrdU-positive cells in E12.5 forebrain regions. The graphical results represent mean ± SEM (control vs. nCT in CTX: 216±36 vs. 198±9; in LGE, 151±17 vs. 100±9; in MGE, 167±18 vs. 86±13). **, p<0.01, Student’s <i>t</i> test. E1–E2’: The Ki67-positive germinal zone was decreased in the nCT brain (E2) compared to the nC brain (E1). The bracketed regions in E1 and E2 are shown with DAPI counter staining at high magnification in E1’ and E2’, respectively. The white double-headed arrows indicate the width of Ki67-positive germinal zone. The arrows in E2 indicate radial lines that are devoid of Ki67-positive cells in the germinal zone of LGE and MGE. Scale bars in A1, B1, C1, E1’ for A1–2 B1–2, C1–2, E1’-2′, respectively, 100 µm; in E1 for E1–2, 150 µm.