Decreased peripheral Foxp3⁺ Tregs in both intact NIK KO and NIK KO single BM chimeric mice.

<p>Single cell suspensions of nucleated splenocytes from intact (A-F) or BM chimeric (G-L) mice were stained with the indicated fluorescently labeled antibodies. A, Plots are gated on CD4⁺; numbers indicate percent of gated cells. B and E, Quantitation of percent of Foxp3⁺ and CD4⁺ splenocytes, respectively. C, D, and F, Quantitation of absolute number of Foxp3⁺, total, and CD4⁺ splenocytes. G, Plots are gated on CD4⁺CD45.1^- cells (BM-derived, NIK KO or WT littermate). Numbers indicate percent of gated cells. H and K, Quantitation of percent of Foxp3 ⁺ CD45.1^- and CD4⁺CD45.1^- cells, respectively. I, J, and L, Quantitation of absolute number of Foxp3 ⁺ CD45.1^-, total CD45.1^-, and CD4⁺CD45.1^- splenocytes, respectively. Data in bar graphs represent mean ± SEM of n=3-4 mice per group in one representative experiment of two (B-F) or three (G-L).