Investigation of the mitochondrial morphology following the addition of fluo-PHB and CCCP.

<p>A) fluo-PHB induced mitochondrial membrane depolarization occurs prior to mitochondrial swelling. HeLa cells were transiently transfected with GFP and then loaded with TMRM and fluo-PHB. Arrows point at the region which lost membrane potential but the shape of mitochondria was not changed compared to polarized mitochondria. Images were collected immediately after loading and at 400 s after addition of the Fluo-PHB; B) experimental conditions are similar to those shown in panel A) except in the lower panel the cells were imaged immediately following the addition of 10 µM CCCP. C) HeLa transiently transfected with pMito-GFP were treated with fluo-PHB (18 ng/ml) and after 400 s these cells were treated with ferutinin (25 µM) showing typical swelling of the mitochondria. Scale bar is 20 µm.