Detection of anti-AQP1 antibodies by an ELISA with immobilized purified human yeast-expressed AQP1.

<p>Sera previously tested by RIPA for AQP1 antibodies were tested for binding to immobilized AQP1 by ELISA. First column contains 31 sera found positive by the RIPA (including a double-positive anti-AQP1/AQP4, empty square, and 3 sera from anti-AQP1-positive MS patients, empty circles). The following 3 columns contain 5 anti-AQP4-positive/anti-AQP1-negative sera, 30 sera from anti-AQP1-negative MS patients and 44 sera from healthy controls). The dashed horizontal line denotes the cut-off (O.D.450: 0.36) for positive values.