DNA lesions induced by CBP in HEp2 and 7T cells.

<p>(A) Upon treatment with 40 µg/mL CBP the cells were collected at the indicated time points and DNA was isolated and transferred on nitrocellulose membrane. After fixation the membrane was incubated with antibody specifically recognizing 1,2-GG intrastrand cross-links induced by CBP. (B) The logarithmically growing cells were treated during indicated time points with 40 µg/mL CBP. The phosphorylation of H2AX (γH2AX) was investigated by Western blot analysis. Expression of ERK2 was used as an internal loading control. Representative data of two independent experiments are presented. Control cells were collected 24 h after the beginning of the treatment.